Psba promoter induction
WebApr 1, 2024 · The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. WebSep 22, 2004 · The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces …
Psba promoter induction
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WebMay 2, 2011 · Here, we examine the effect of fusing the strong 16S rRNA promoter to the … Webmature chloroplasts, the promoters of psbA and psbD are predominantly recognized by …
Webtested an ethanol inducible native alcA promoter from Aspergillus nidulans in Escherichia … WebJan 1, 2014 · In this protocol we will show how to transform, screen, and monitor …
WebNov 8, 1998 · Furthermore, a series of promoter fragments were constructed to confirm minimal cis elements for the in vitro psbA2 transcription. A -80 to +6 or -38 to +46 region, the sequences of which consisted of a core promoter (-38 to +6), was identified as the potential minimal cis element using the RNA polymerase fraction (*EsigmaA1) containing sigmaA1 ... WebFeb 19, 2024 · The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After...
WebMay 3, 2013 · CEC2 utilized the read-through transcription for expression of xynA, while the psbA gene promoter (PpsbA) along with its 5’ UTR governed the expression of aadA. CEC3 was the only cassette designed to contain two strong chloroplast promoters, P psbA and mutated P rrn (mP rrn, see Methods for a description of the mutations; [ 45 ]), driving the ...
WebIn mature chloroplasts, the promoters of psbA and psbD are predominantly recognized by AtSIG5 among six sigma factors. Using a protoplast transient expression assay with AtSIG5–AtSIG1 chimeric genes, we present evidence that AtSIG5 contains determinants for activating the psbD blue-light-responsive promoter (BLRP) in region 4.2 rather than ... denver news stations tvWebSep 22, 2024 · Induction of a group 2 σ factor, RPOD3, by high light and the underlying mechanism in Synechococcus elongatus PCC 7942. J. Biol. Chem. 2007; 282 ... desirable in further analysis of such regulatory pathways. Nonetheless, direct binding assays of sigma factors with psbA promoter regions can provide immediate insight into their … fg tws y1denver news weather 7WebJul 14, 2024 · The psbA promoter was used to drive constitutive expression of bar, which confers resistance to phosphinothricin, followed by the regulatory element, rbcL terminator. The rbcL promoter was used to drive the expression of the target gene, followed by the MCS (multiple cloning site) and the regulatory element, psbA terminator. The MCS provides ... denver news stations liveWebThe purpose of this study was to express an antimicrobial peptide in the chloroplast to further develop the plastid engineering of H. pluvialis. Homologous targeting of the 16S-trnI/trnA-23S region and four endogenous regulatory elements, including the psbA promoter, rbcL promoter, rbcL terminator, … denver news weather girlsWebApr 3, 2007 · It has been reported that the principal σ factor SigA can recognize the psbA promoters for basal transcription, and SigD contributes to the light-induced transcription of psbA under light in PCC 6803 cells [9], ... Nitrogen induction of sugar catabolic gene expression in Synechocystis sp. PCC 6803. DNA Res., 13 (2006), pp. 185-195. fgt when we were youngWebNov 8, 1998 · Abstract. The psbA2 gene of a unicellular cyanobacterium, Microcystis … fgtv youtube toys