Polyhydroxybutyrate depolymerase
A total of 79 bacterial strains were successfully isolated from the wastewater treatment area of Kasetsart University, Bangkok, Thailand. Nile red agar plates were used for preliminary screening to select PHB-producing strains. Ten strains exhibited bright orange fluorescence under UV light after being incubated … See more To identify BSRT1-1, the 16S rRNA gene of strain BSRT1-1 was extracted and sequenced. The sequence of the 16S rRNA gene (1,483 bp) was obtained and used … See more PHA biosynthesis was performed in a 250-mL flask to evaluate PHB production in R. pyridinivorans BSRT1-1 and to select the best carbon and nitrogen … See more A three-variable-five-level design of CCD was used to determine the optimized medium composition for PHB accumulation and the interactive effects of each … See more To enhance the biomass and PHB accumulation of R. pyridinivorans BRST1-1, batch cultivation was carried out in a 10 L stirred-tank bioreactor containing 6 … See more WebThis work proposes the purification of an active and efficient enzyme, extracellular poly(3-hydroxybutyrate) (PHB)-depolymerase, suitable for industrial applications. This is achieved by the application of an easy, fast, and cheap route, skipping the chromatography step. Chromatography with one or two columns is a common step in the purification procedure, …
Polyhydroxybutyrate depolymerase
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WebMar 1, 2024 · Polyhydroxybutyrate degrading biocatalysts were screened from the sludge sample. • Bacteria degraded PHB from the wastewater. • Co-culture of Nitrobacter and Pseudomonas improved PHB degradation. • 96% degradation was achieved after 8 days in the sequencing batch biofilm reactor. • Polyhydroxybutyrate depolymerase influenced on … WebTwo methods for accurate poly(3-hydroxybutyrate) (PHB) depolymerase activity determination and quantitative and qualitative hydrolysis product determination are described. The first method is based on online determination of NaOH consumption rates necessary to neutralize 3-hydroxybutyric acid (3HB) and/or 3HB oligomers produced …
WebBackground Poly-β-hydroxybutyrate (PHB) is the most important and versatile class of biodegradable polymers used successfully in the medical, agricultural and industrial field. Idea is to find the novel isolate for degradation of biodegradable plastics that can enhance the bioremediation. Materials and methods Thirty-one PHB and PHB depolymerase … WebIn this study, we constructed a set of single-, double-, and triple-gene deletion strains of Ralstonia eutropha H16 in (p)ppGpp synthase/hydrolase (spoT1), (p)ppGpp synthase (spoT2) and polyhydroxybutyrate (PHB) depolymerase (phaZa1 or phaZa3) genes
WebMar 1, 2024 · The bacteria produced depolymerase and enzyme activity was maximum after 72 h. The sequencing batch biofilm reactor (SBBR) co-cultured with N. vulgaris SW1 and P. aeruginosa KS10 was operated to remove PHB from the wastewater. Biofilm in the reactor degraded PHB and the production of polyhydroxybutyrate depolymerase influenced on … WebAug 1, 2024 · DOI: 10.1016/J.POLYMDEGRADSTAB.2024.06.002 Corpus ID: 89893246; Purification, characterization, and gene cloning of an Aspergillus fumigatus polyhydroxybutyrate depolymerase used for degradation of polyhydroxybutyrate, polyethylene succinate, and polybutylene succinate
WebSingle crystals of bacterial poly[(R)-3-hydroxybutyrate] (P(3HB)) with different molecular weights (Mn = 2500 and Mn = 48 000) were prepared in a mixture of chloroform and …
WebMay 1, 2015 · In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarchaeon Haloferax mediterranei. PhaZh1 hydrolyzed the native PHA (nPHA) [including native polyhydroxybutyrate (nPHB) and native poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (nPHBV) in this study] granules in vitro with … birthday message for grandmother far awayWebWe demonstrate a novel strategy to produce ( R )-3-hydroxybutyric acid [( R )-3-HB] using Pseudomonas sp. DS1001a or its extracellular poly (3-hydroxybutyrate) (PHB) depolymerase. While approximately 77.8% PHB was degraded by Pseudomonas sp. DS1001a after 12 h of cultivation, only 1.555 g/l of 3-HB monomer was produced because … danny thomas obituaryWebWarning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data danny thomas meets andy griffith episodeWebAn extracellular polyhydroxybutyrate depolymerase in Thermus thermophilus HB8. Applied Microbiology and Biotechnology. 2009 Jun;83(4):659-668. doi: 10.1007/s00253-008-1842-2. Powered by Pure, Scopus & Elsevier Fingerprint Engine ... danny thomas golf tournamentWebJun 18, 2024 · Poly-β-hydroxybutyrate (PHB) depolymerase is known to decompose PHB, biodegradable polymers and therefore has great commercial significance in the bioplastic … birthday message for grandmaWebTwo methods for accurate poly (3-hydroxybutyrate) (PHB) depolymerase activity determination and quantitative and qualitative hydrolysis product determination are … birthday message for grown sonWebJun 17, 2016 · Petroleum polymers contribute to non-degradable waste materials and it would therefore be desirable to produce ecofriendly degradable materials. Biodegradation of polyhydroxybutyrate (PHB) in the presence of oligomer hydrolase and PHB depolymerase gave 3-hydroxybutyric acid which could be oxidized to acetyl acetate. Several bacteria and … danny thomas hospital legacy