Tīmeklis2024. gada 24. nov. · Lamp2b-HuR融合蛋白功能化的外泌体能够将被RNA结合基序识别的RNA靶标驱动至溶酶体以在受体细胞中降解。 该系统在富含溶酶体的巨噬细胞中 … Tīmeklis2024. gada 31. janv. · Lamp2b was cloned with cDNA from C2C12 cells and XhoI and BspEI restriction sites were inserted after the signal peptide sequence together with …
Stabilization of exosome-targeting peptides via engineered ...
http://www.biofeng.com/adjy/zhili/pcDNA%20Flag%20Lamp2b-HA.html Tīmeklis2024. gada 30. apr. · The transfection of the dendritic cells with the Lamp2b-RVG plasmid is necessary to obtain brain-targeted exosomes. Use a different targeting peptide to direct exosomes to other organ targets. Alternatively to produce non-targeted exosomes just replace the medium on day 4 with 3 mL medium supplemented with … download pdf from google play books
JEV:融合蛋白工程化的外泌体靶向降解特定RNA - 外泌体资讯网
Tīmeklis2024. gada 8. janv. · Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome-lysosome fusion in human cardiomyocytes (CMs). Remarkably, LAMP-2B functions independently of syntaxin 17 (STX17), a protein that is essential for autophagosome-lysosome fusion in non-CMs. Instead, LAMP-2B interacts with … Tīmeklis2011. gada 20. marts · Expression of the RVG and MSP Lamp2b constructs was confirmed by quantitative PCR (qPCR) analysis of transfected dendritic cells ( Fig. 1e … Tīmeklis一种基于Lamp2b的融合蛋白可用于在表面显示狂犬病病毒糖蛋白(RVG),当这些胞外体被系统地给予时,表面展示的RVG导致这些胞外体在小鼠的神经元和大脑中积聚 … download pdf from embedded viewer