Biotin-coupled mirna capture
WebFeb 12, 2024 · Biotin-coupled miRNA capture assay. The biotin-labeled circHIPK3 and control probes were synthesized by GenePharma (Shanghai, China). In brief, biotin-labeled circHIPK3 and control probes were … WebAug 7, 2024 · The capture probe, 35 to 50 base-long and coupled to biotin on its 3′ end, binds the 5′ half of the miRNA. The detection probe is composed of a 3′ end complementary to the miRNA and a chain of fluorescently labeled RNA sequences, forming a fluorescent barcode associated with the target miRNA.
Biotin-coupled mirna capture
Did you know?
WebJul 17, 2024 · Biotin-coupled miRNA capture. Because PTEN plays an important role in regulating cell proliferation and is one of the miR-181b-5p target genes, a pull-down assay was performed to investigate whether miR-181b-5p directly binds to PTEN. Briefly, HPDLSCs were transfected with biotinylated miRNA mimics or nonsense control (NC) … WebJan 31, 2024 · Biotin-coupled probe pull down assay, Biotin-coupled miRNA capture, Fluorescence in situ hybridization and Luciferase reporter assay were conducted to confirm the relationship between the circ-ITCH and the microRNA. Results: In the present study, we found that circ-ITCH, is down-regulated in BCa tissues and cell lines. BCa patients with …
WebJan 31, 2024 · The biotin-coupled RNA complex was pull-downed by incubating the cell lysates with streptavidin magnetic beads (Life Technologies, USA) for another 4 h. The … WebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ...
WebIn the biotin-coupled miRNA capture assay, the direct interaction between HIPK3 and miR-194 was verified. While upregulating HIPK3 promoted NSCLC cell proliferation, co-transfection with both HIPK3 and miR-194 abrogated the tumor-promoting effects of HIPK3. Altogether, these data corroborated that HIPK3 and miR-149 exert opposing roles in … WebFeb 19, 2024 · Provided herein are methods of identifying the spatial location of a nucleic acid in a biological sample. In some embodiments, the methods employ a template switching oligonucleotide. In some embodiments, the methods extend the capture probe to create a complementary DNA (cDNA) molecule of a capture analyte and produce second strand …
WebJan 16, 2024 · RNA immunoprecipitation (RIP) and biotin-coupled miRNA capture. g for 20 min. Western blot analysis. The total protein of 95D cells were exacted using protein extraction reagent (Thermo Scientific ...
WebFeb 10, 2024 · Biotin-coupled miRNA capture. The 3′ end biotinylated miR-578 or control mimic RNA (RiboBio) was transfected into RF/6A cells for 24 h at the concentration of 30 nM. The biotin-conjugated RNA complex was pulled down by incubating the cell lysates with streptavidin-coated magnetic beads (Life Technologies). The amount of cZBTB44 in … floor vents heating square footageWebInvitrogen streptavidin-coupled Dynabeads are a robust and versatile tool that can be used to capture specific RNA or DNA sequences and then pull them directly out of solution. … floorvibe downloadWebNov 9, 2024 · On the contrary, some studies argued that biotin moiety when attached to the 3′ end of the miRNA impede its association with AGO2. 41 Hence, biotin-labelled miRNA was not co-IPed with AGO2. 28 The negative result could be due to low transfection efficiency or post-translational modification of AGO2 that can hamper miRNA-AGO2 … floor vent for bathroomWebDec 20, 2024 · Biotin-coupled miRNA capture. For biotin-coupled capture, approximately 5 × 10 6 BCa cells (T24 and 253 J-BV) were transfected with biotin-miR135a-5p or negative control (NC) by … great reparation offices都灵时装周WebJul 19, 2024 · Bioinformatic analysis was utilized to predict potential miRNA target sites, and biotin-coupled miRNA capture, biotin-coupled probe pull-down assay, and RNA … great renunciation buddhaWebDec 12, 2024 · Streptavidin coupled to a solid matrix of beads allows the specific capture of biotinylated RNA easily. To demonstrate the suitability of biotinylated cap analogues for the study of protein–RNA interactions we established a combined protocol including ultraviolet (UV) cross-linking, and affinity purification of biotinylated-capped RNA. floor venue blacktown reviewWebJan 10, 2024 · Biotin‐coupled miRNA capture The 50 μ m biotinylated miRNA mimic (GenePharma, Shanghai, China, sequence GCCCTTCATGCTGCCCAG) was … floorvibe software